Journal: Frontiers in Oncology

Article Title: Deciphering the let-7c-5p/RRM2 axis in lung adenocarcinoma: expression, prognosis, and immune landscape implications

doi: 10.3389/fonc.2025.1628429

Figure Lengend Snippet: RRM2 expression in LUAD and its clinical implications. (A, B) The GEO dataset and TCGA-LUAD dataset download from the BEST database showed that RRM2 was highly expressed in LUAD. (C) Pathologic stage. (D) Pathologic T stage. (* p <0.05; ** p <0.01; *** p <0.001, ns, no significance).

Article Snippet: The primary antibodies for RRM2 polyclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Deciphering the let-7c-5p/RRM2 axis in lung adenocarcinoma: expression, prognosis, and immune landscape implications

doi: 10.3389/fonc.2025.1628429

Figure Lengend Snippet: Prognostic value of RRM2 in LUAD. (A) Overall survival analysis of RRM2 mRNA high and low expression in LUAD. (B) Pps analysis of RRM2 mRNA high and low expression in LUAD. (C) FP analysis of RRM2 mRNA high and low expression in LUAD. (D) Survival curves of high and low RRM2 expression in the GsE68571. (E) The KM survival curve of the gene in TCGA data. (F) Time-dependent ROC curve. (G) Univariate and (H) multivariate COX regression analysis of OS correlation in LUAD. Fp, First Progression; OS, Overall Survival; PpS, Post Progression Survival.

Article Snippet: The primary antibodies for RRM2 polyclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Deciphering the let-7c-5p/RRM2 axis in lung adenocarcinoma: expression, prognosis, and immune landscape implications

doi: 10.3389/fonc.2025.1628429

Figure Lengend Snippet: Identification of let-7c-5p as a potential upstream regulatory miRNA of RRM2 in LUAD. (A) The miRNA-RRM2 prediction network produced by Cytoscape. (B) The correlation between the predicted expression of some candidate miRNAs and RRM2 in LUAD analyzed by starBase. (C, D) The expression and prognostic value of let-7c-5p in LUAD were detected by starBase and Kaplan Meier plotter.

Article Snippet: The primary antibodies for RRM2 polyclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Produced, Expressing

Journal: Frontiers in Oncology

Article Title: Deciphering the let-7c-5p/RRM2 axis in lung adenocarcinoma: expression, prognosis, and immune landscape implications

doi: 10.3389/fonc.2025.1628429

Figure Lengend Snippet: The effect of let-7c-5p regulation on RRM2 in LUAD. (A) The let-7c-5p expression in A549 and H460 cells after let-7c-5p mimic/control transfection was determined by RT-qPCR. (B) RRM2 siRNA transfection efficiency was detected by qRT-PCR. (C, D) Proliferation of A549 and H460 cells after let-7c-5p transfection determined by the CCK8 assay. (E) Let-7c-5p mimic could decrease the expression of RRM2. (F) Blocking RRM2 expression could decrease the metastasis number of A549 and H460 cells (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: The primary antibodies for RRM2 polyclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Expressing, Control, Transfection, Quantitative RT-PCR, CCK-8 Assay, Blocking Assay

Journal: Frontiers in Oncology

Article Title: Deciphering the let-7c-5p/RRM2 axis in lung adenocarcinoma: expression, prognosis, and immune landscape implications

doi: 10.3389/fonc.2025.1628429

Figure Lengend Snippet: Association between RRM2 and immune cell infiltration in LUAD. (A, B) According to different expression levels of RRM2, the infiltration levels of immune cells were analyzed in groups. (C-O) RRM2 is correlated with immune infiltration in LUAD. (P) The relationship between the altered somatic copy number of RRM2 gene and infiltrating immune cells in LUAD (* p < 0.05; ** p < 0.01; *** p < 0.001, ns, no significance).

Article Snippet: The primary antibodies for RRM2 polyclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Deciphering the let-7c-5p/RRM2 axis in lung adenocarcinoma: expression, prognosis, and immune landscape implications

doi: 10.3389/fonc.2025.1628429

Figure Lengend Snippet: Single-cell transcriptomic characterization of RRM2 expression in immune cell subsets of LUAD. (A) Cell type lineage mapping. (B) Density map of RRM2 expression projected onto the t-SNE embedding. (C) Mean variance relationship of gene expression levels. (D) Violin plot depicting RRM2 expression across major T-cell subsets.

Article Snippet: The primary antibodies for RRM2 polyclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: Expressing, Gene Expression

Journal: Frontiers in Oncology

Article Title: Deciphering the let-7c-5p/RRM2 axis in lung adenocarcinoma: expression, prognosis, and immune landscape implications

doi: 10.3389/fonc.2025.1628429

Figure Lengend Snippet: The schematic diagram of let-7c-5p/RRM2 in LUAD.

Article Snippet: The primary antibodies for RRM2 polyclonal antibody were purchased from Cell Signaling Technology (Danvers, MA, United States).

Techniques: